Comparative study on Hydroxyapatite extraction from bovine and caprine bone

Project Title

Comparative study on Hydroxyapatite extraction from bovine and caprine bone

Project Area of Specialization Shared EconomyProject Summary

Statement of purpose

Objectives:

Ø Extraction of hydroxyapatite from Bovine and Caprine bone.

Ø Characterization of hydroxyapatite by using XRD and FTIR.

Ø Comparison of results on the basis of molarity, temperature and time.

 Motivation:

The biomaterial is an emerging and challenging field in the healthcare sector. It has several applications in medicine such as prosthetics, orthopedics, dentistry, tissue engineering, and drug delivery system. We are fascinated to work in this field as our final year project. Working in the domain of biomaterials, we initiate our work based on “Comparative study on Hydroxyapatite extraction from Bovine and Caprine bone”. Hydroxyapatite has widely been used in various applications due to their excellent biocompatibility, bioactivity and osteoconduction characteristics. This material may be employed in many forms such as porous blocks, dense body, granular forms and hybrid composites for various biomedical applications..

Materials and devices required:

Materials

• Bones (Bovine and Caprine).

• Deionized water

• Sodium hydroxide

• Hydrogen peroxide

• Acetone

Machine and devices

• Automatic furnace

• Water bath

• Oven

• pH meter

Methodology

Ø Cleaning of raw bones.

Ø Chemical treatment of clean bones.

Ø Thermal treatment of samples.

Ø Characterization of the final product.

Result

The use of bones for the extraction of hydroxyapatite provide an environmentally beneficial and cost-effective medical grade biocompatible material. The characterization will confirm the purity and crystallinity of the final product.

Project Objectives

OBJECTIVES:

Ø Extraction of hydroxyapatite from Bovine and Caprine bone.

Ø Characterization of hydroxyapatite by using XRD and FTIR.

Ø Comparison of results on the basis of molarity, temperature and time.

Project Implementation Method

Starting from the collection of bones we boiled the bones with water to remove the muscular attachments and then cleaned the bones with a chemical solution(acetone & deionized water). then we divided bones into 10 samples and treat them with different concentration of chemicals. After chemical treatment, we calcinate the samples using the automatic furnace. In the last samples were grinded to reduce the particle size.

Benefits of the Project

• Bone tissue engineering.
• Coating of dental implants.
• Re-mineralizing agent in toothpaste
• Filtration of microorganisms.
• In orthopaedics, fillers for bone voids
• filler for maxillofacial and dental surgery
• Repairing of periodontal disease and porous bone.
• Drug and gene delivery.

Technical Details of Final Deliverable

Technical details of final deliverable:

Bones of cow and goat were collected as a raw material., beakers for holding the samples that were dipped in a chemical solution. Evaporated plates used for calcination.Motor and pestle for grinding the samples. Airtight bags used for storage of samples. FTIR, XRD & SEM test of samples for characterization.

Final Deliverable of the Project Hardware SystemType of Industry Manufacturing Technologies Shared EconomySustainable Development Goals Responsible Consumption and ProductionRequired Resources

Item Name	Type	No. of Units	Per Unit Cost (in Rs)	Total (in Rs)
			Total in (Rs)	28940
Caprine bones	Miscellaneous	10	50	500
Bovine bones	Miscellaneous	10	100	1000
Beakers	Miscellaneous	10	40	400
Evaporated plates	Miscellaneous	15	35	525
airtight bags	Miscellaneous	35	3	105
Motor and pestle	Miscellaneous	1	500	500
Stationary	Miscellaneous	4	60	240
Priting	Miscellaneous	38	30	1140
FTIR test	Equipment	20	300	6000
XRD test	Equipment	20	500	10000
SEM test	Equipment	20	300	6000
Transport(auto)	Miscellaneous	9	170	1530
Transport(karachi)	Miscellaneous	2	500	1000